Estimates suggest that pulpal disease may affect up to 30% of the world’s population. Up to 14% of endodontics therapy ends in infections within the root canal system and is difficult to treat. This project aims to establish a novel approach using nanobubble water combines with apyrase during endodontic therapy which focuses on preventing biofilm formation and reducing bacterial colonization which consequently minimizes the risk of infections post endodontics therapy. The objectives of this proposal will have a significant impact on public health by providing promising approach for endodontics therapy without changing existing endodontic procedure. We expect to decrease the post endodontics infections to less than 5% by using our novel approach.